A new open-source device allows scientists to identify treatments and conditions that affect the mechanical properties of plant cells
Determining how various treatments and conditions affect the mechanical properties of plant cells could allow scientists to understand plant growth at the cellular level and devise ways to enhance it. In a breakthrough report published in The Plant Cell, a team of researchers introduces an innovative robotic tool that measures the mechanical properties of plant cells with cellular resolution.
Plant scientists have a new tool in their toolkit. The automated confocal microextensometer (ACME) developed by a team of researchers in Europe and the US—allows scientists to measure spatial variation in the mechanical properties of plant cells with unprecedented accuracy. Plant cell growth is limited by the mechanical properties of the surrounding cell wall. Cell walls in the growing parts of a plant are thought to be much more extensible (stretchy) than those in mature parts, and these local differences in cell wall extensibility affect the overall shape of the plant.
Until now, it was not possible to measure cell wall extensibility in the direction of growth in living plants. A team of researchers led by Cris Kuhlemeier of the Institute of Plant Sciences at the University of Bern in Switzerland created a system that does just that.
“Intuitively the simplest way to do this is to stretch the plant and look at how much each cell stretches,” explains first author Sarah Robinson. Using this principle, the researchers cobbled ACME in action.
Researcher Sarah Robinson setting up ACME in the lab. together a robotic pipeline that combines custom and commercially available parts. They designed a specialized device that holds plants in place without damaging them and then stretches them under a defined pull. The device, which is mounted on a microscope, is controlled by software that allows the user to specify the duration and degree of stretching. High-resolution images of the plant are taken during stretching, and custom-built software uses the images to compute mechanical properties in different regions of the plant.
The authors named the device ACME after the fictitious corporation featured in the Road Runner/Wile E. Coyote cartoons, because they were inspired by the tireless efforts of Wile E. Coyote. “Thankfully, in the end, we were more successful, but some of our prototypes were less elegant and involved a lot of scotch tape,” jokes Sarah.
Using ACME, the authors demonstrated that cells in the stems of seedlings exhibit a gradient of mechanical properties in the presence of the plant growth hormone gibberellic acid. Furthermore, they used their versatile system to show that stretching induces irreversible increases in cell length in living plant cells, but that the increases in cell length are partially reversed in dead plant tissues once stretching stops. While ACME was built to accommodate small samples—the specimen used in this study was a tiny weed known as thale cress—it can readily be adapted for use with larger samples and different imaging systems.
The team has made all the information needed to create this system freely available, so that plant scientists in other labs can use ACME in their own research. “ACME has many applications and scope for future use,” says Sarah Robinson. For instance, this device can help us understand the mechanisms by which various inputs and treatments alter plant growth at the cellular level. Furthermore, it might elucidate conditions and treatments that promote plant cell wall extensibility, and thus enhance plant growth at the cellular level. Given the escalating demands for biomass production, this is a very exciting prospect.
Kathleen L. Farquharson, PhD email@example.com
Science Editor, The Plant Cell
This work was supported by SystemsX.ch project ‘Plant Growth in a Changing Environment’ [SXRTX0-123956 and 51RT0-145716 to C.K.]. SR received an EMBO long term fellowship. SB was funded by The Gatsby Charitable Foundation (GAT3396/PR4) and the BBSRC (BB.L002884.1).
Full citation: Sarah Robinson, Michal Huflejt, Pierre Barbier de Reuille, Siobhan A. Braybrook, Martine Schorderet, Didier Reinhardt, and Cris Kuhlemeier. (2017). An automated confocal microextensometer enables in vivo quantification of mechanical properties with cellular resolution. Plant Cell 10.1105/tpc.17.00753.
About the researchers: To arrange an interview with a member of the team that conducted this study, please contact Dr. Cris Kuhlemeier of the Institute of Plant Sciences, University of Bern, Bern, Switzerland at firstname.lastname@example.org.
About The Plant Cell: Published monthly by ASPB, The Plant Cell (http://www.plantcell.org/) is the highest-ranking primary research journal in plant biology. The Plant Cell publishes novel research in plant biology, especially in the areas of cellular biology, molecular biology, genetics, development, and evolution. The primary criteria for publication are that the article provides new insight that is of broad
interest to plant biologists, not only to specialists, and that the presentation of results is appropriate for a wide audience.
About ASPB: ASPB is a professional scientific society, headquartered in Rockville, Maryland, devoted to the advancement of the plant sciences worldwide. With a membership of almost 5000 plant scientists from throughout the United States and more than 50 other nations, the Society publishes two of the most widely cited plant science journals: The Plant Cell and Plant Physiology. For more information about ASPB, please visit http://www.aspb.org/. Also, follow ASPB on Facebook at facebook.com/myASPB and on Twitter @ASPB.
Figure credit: Cris Kuhlemeier, University of Bern.
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Keywords: biomass, open-source, plant growth, plant science, robotic device