This week’s Research in Focus article has two take-home messages. The first is about how an important plant pathogen is recognized by its host: specifically, the role of a newly-identified tyrosine-sulfated bacterial protein. The second is about the process of science and the foundation of trust on which it rests: specifically, how to proceed when an error is recognized.
Discovery of the activator for rice immune receptor XA21
Xanthomonas oryzae pathovar oryzae (also known as Xoo) is a bacterial pathogen of rice. By studying rice strains resistant to Xoo, members of Pamela Ronald’s lab mapped resistance to XA21, a leucine-rich repeat receptor kinase (Song et al, 1995). Until recently, the question of what this receptor recognizes to initiate resistance was uncertain. The lab thought they had the answer in 2009, but later found that there were errors in their studies, leaving the receptor’s activator an open question (Lee et al., 2013).
In a new paper by Pruitt et al., (2015), a small bacterial protein known as RaxX was shown to be necessary and sufficient for recognition by XA21. One of the interesting things about RaxX is that it is recognized only when one of the tyrosine residues is sulfated; bacterial strains that fail to produce RaxX or that cannot sulfate it, as well as some strains isolated from rice-growing areas in India that carry a variant RaxX allele, are able to avoid detection by XA21.
Bacterial tyrosine-sulfated proteins were unknown until recently, and this is the first identification of a bacterial protein with this modification. Interestingly, tyrosine-sulfated proteins are produced in plants, including some with roles in defense (see Mosher et al., 2013). As yet the biological function of RaxX is not known. Does it confer some benefit to Xoo (in the absence of its detection by the receptor XA21), perhaps mimicking the properties of an endogenous plant protein?
By identifying the protein recognized by XA21, this research opens up possibilities for engineering receptors with broader recognition capabilities and for extending resistance to Xanthomonas pathogens of crops beyond rice.
Lessons about the process of science
In the guide to responsible conduct in research, the National Academy’s Committee on Science, Engineering and Public Policy states that “The scientific enterprise is built on a foundation of trust” (2009). Two of the core responsibilities of a research scientist are to report results factually and to report mistakes when they are identified. The steps taken by the Ronald lab after they realized that their 2009 publication contained errors provides an exemplar for how these responsibilities should be carried out in practice. First-person accounts from the researchers about the process they undertook can be found in Schwessinger et al., (2015), Ronald (2013), and Retraction Watch (2015). I urge everyone to read these accounts and the NAS guide, and to remember that the collective privilege of seeking knowledge comes with the collective personal responsibility of research integrity.
Committee on Science, Engineering and Public Policy (2009). On being a scientist: A guide to responsible conduct in research. 3rd ed. National Academies Press. (Free ebook http://www.nap.edu/catalog/12192/on-being-a-scientist-a-guide-to-responsible-conduct-in).
Lee, S.-W., Han, S.-W., Sririyanum, M., Park, C.-J., Seo, Y.-S. and Ronald, P.C. (2013). Retraction. Science. 342: 191.
Mosher, S., Seybold, H., Rodriguez, P., Stahl, M., Davies, K.A., Dayaratne, S., Morillo, S.A., Wierzba, M., Favery, B., Keller, H., Tax, F.E. and Kemmerling, B. (2013). The tyrosine-sulfated peptide receptors PSKR1 and PSY1R modify the immunity of Arabidopsis to biotrophic and necrotrophic pathogens in an antagonistic manner. Plant J. 73: 469-482.
Pruitt, R.N., Schwessinger, B., Joe, A., Thomas, N., Liu, F., Albert, M., Robinson, M.R., Chan, L.J.G., Luu, D.D., Chen, H., Bahar, O., Daudi, A., De Vleesschauwer, D., Caddell, D., Zhang, W., Zhao, X., Li, X., Heazlewood, J.L., Ruan, D., Majumder, D., Chern, M., Kalbacher, H., Midha, S., Patil, P.B., Sonti, R.V., Petzold, C.J., Liu, C.C., Brodbelt, J.S., Felix, G. and Ronald, P.C. (2015). The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium. Sci. Adv. 1: e1500245.
Retraction Watch (2015). What do you do after painful retractions? Q&A with Pamela Ronald and Benjamin Schwessinger. http://retractionwatch.com/2015/07/24/qa-with-pamela-ronald-redemption-after-retraction/
Ronald, P. (2013). Lab life: The anatomy of a retraction. Scientific American. October 10, 2013.
Schwessinger, B., Ronald, P., Pruitt, R., Joe, A., and Bahar, O. (2015). A Phoenix rises from the ashes: A new discovery emerges from the 2009 retraction. In The Tree of Life, blog of Jonathan Eisen. http://phylogenomics.blogspot.co.uk/2015/07/a-phoenix-rises-from-ashes-new.html.
Song, W.-Y., Wang, G.-L., Chen, L.-L., Kim, H.-S., Pi, L.-Y., Holsten, T., Gardner, J., Wang, B., Zhai, W.-X., Zhu, L.-H., Fauquet, C. and Ronald, P. (1995). A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science. 270: 1804-1806.
This post is part of our “Research in Focus” series, highlighting significant plant science breakthroughs and discoveries from around the globe, by Mary Williams (https://twitter.com/PlantTeaching). If you have ideas or suggestions please email firstname.lastname@example.org, using Research in Focus as the subject, and include a link as well as an explanation of why this should be highlighted.